Effects of flower extract from Nelumbo nucifera on high-K+ & Ca2+-induced colonic contraction in diabetic rats

Nopparat Buddhakala, Supasorn Sakuljaitrong

Abstract


Gastrointestinal disorder has been commonly found in diabetic patients. An increase responsiveness to electrical responsiveness to neurotransmitters has been reported. N. nucifera has been used for the treatment of diabetes. The aim of this study was therefore to investigate the effect of N. nucifera flower extract (NNFE) on high K+ and Ca2+-ion induced colonic contraction in diabetic rats. Male albino wistar rats were induced with 65 mg/kg b.w. streptozotocin. All groups of rats were daily oral administered of NNFE (250 mg/kg b.w.) for 8 weeks. The rats were sacrificed by asphyxiation with CO2. Longitudinal colonic smooth muscle strips of the rats were prepared for SNP-responsive investigation. The results showed that NNFE significantly (p<0.05) reduced the contractile responses of colonic smooth muscle strips of the rats in the presence of K+ and Ca2+-ion. And also, NNFE significantly (p<0.05) reduced the responses of the strips in the presence of SNP. The changes in intracellular Ca2+ concentrations lead to fusion of insulin-containing vesicles to the plasma membrane and subsequent rapid exocytosis of insulin from β cells. The data indicated that the flower extract from N. nucifera posses favorable hypoglycemic activity and inhibited colonic contractile activity in diabetic rats.

Keywords


Nelumbo nucifera, Hypoglycemic activity, Diabetic rats, Colonic contraction

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