Suitable cell age for enhanced poly-ß-hydroxybutyrate accumulation under photoautotrophic nutrient deprivation of Synechocystis sp. PCC 6803

Wanthanee Khetkorn, Suttawan Suphan, Sirikhae Pongswat

Abstract


Synechocystis sp. PCC 6803 can convert atmospheric CO2 and water into carbohydrates by photosynthesis and store them as glycogen, lipid or poly-ß-hydroxybutyrate (PHB) when nutrient limitation. In this study, under normal photoautotrophic condition, Synechocystis cells could utilize nitrate and phosphate in medium for supporting growth, but cells did not show high PHB accumulation (0.1 – 6.5 %(w/w) DW) in all cultivated times although the concentration of nitrate and phosphate were low levels. The age of cell was found  to have a major effect on PHB accumulation in Synechocystis cell. The maximum PHB content was 15.2 % (w/w) DW when cells at mid-stationary phase adapted in photoautotrophic nutrients (nitrogen and phosphorus) deprivation for 8 days. However, cells in all stationary phase were also found the accumulation of glycogen which higher levels than PHB. Therefore the main carbon storage component under photoautotrophic nutrient deprivation might be glycogen and PHB as a co-component, respectively.


Keywords


Cell age, Poly-ß-hydroxybutyrate, Nutrient deprivation, Synechocystis sp. PCC 6803

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SCIENCE  AND  TECHNOLOGY  RMUTT  JOURNAL
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