Immobilization of poly(L-lactide)-degrading enzyme from Laceyella sacchari LP175: characterization and evaluation for hydrolysis of poly(L-lactide) polymer

Thanasak Lomthong, Saisamorn Lumyong, Alain Marty, Vichien Kitpreechavanich

Abstract


The poly(L-lactide) (PLLA)-degrading enzyme from the thermophilic filamentous bacterium, Laceyella sacchari LP175, was entrapped in calcium alginate beads and characterized using emulsified PLLA as a substrate. The 1.0% sodium alginate appeared most effective for the immobilization, with 60% actual immobilization efficiency and 80% theoretical immobilization efficiency. Immobilized enzyme showed emulsified PLLA hydrolyzing activity similar to free enzyme and it was stable. The immobilized enzyme showed an operational stability up to five times that of the free enzyme, indicating that it is a suitable choice for applications due to reduced enzyme preparation costs. The temperature optima and thermal stability of the immobilized PLLA-degrading enzyme shifted from 60 to 65 oC and 55 to 70 oC, respectively, while the pH optima and stability remained unaltered. The immobilized enzyme showed a higher stability at 60 oC for up to 12 h and improved the lactic acid tolerance ability up to 10% (v/v) as compared to the free enzyme which could help avoid lactic acid feedback inhibition during hydrolysis. Hence, the PLLA-degrading enzyme from L. sacchari LP175 was more stable after immobilization and represents a highly appropriate choice for applications in terms of the recycling process of emulsified PLLA polymer.


Keywords


Poly(L-lactide)-degrading enzyme: Laceyella sacchari LP175: Immobilization: Biodegradation

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