Study on Production of 5´- Phosphodiesterase from Halotolerant Bacillus sp. RMUT 001

Watcharewan Boonsongsri, Anan Boonpan, Sirikhae Pongswat

Abstract


The optimum conditions for growth and 5´ phosphodiesterase (5’- PDE) production from a halotolerant Bacillus sp. RMUT 001 isolated from raw fish sauce in Sehgal and Gibbons Complex (SGC) liquid medium were studied. The results showed that a halotolerant Bacillus sp. RMUT 001 could produce the highest 5´- PDE activity at pH of 7.0 and temperature of  37 oC in SGC liquid medium without NaCl. Cultivation of Bacillus sp. RMUT 001 in shaking flasks at optimum conditions found that enzyme secretion was coupled to cell multiplication and maximum activity was obtained at stationary phase. The maximum 5´- PDE production was 0.98 U/ml after 5 days cultivation. Enzyme was partially purified by 50% ethanol precipitation (by volume). The purity of 5´- PDE was increased 17.79 folds and yield of 73.67% was obtained.

Keywords


5´- phosphodiesterase, Halotolerant bacteria, Bacillus sp.,Raw fish sauc

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SCIENCE  AND  TECHNOLOGY  RMUTT  JOURNAL
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Faculty of Science and Technology
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