Bioactivity test and GC–MS analysis of different solvent extracts from Perilla frutescens (Linn.) Britton and cosmetic product application for sensitive skin

Lapatrada Mungmai, Weeraya Preedalikit, Nattapol Aunsri, Nichakan Peerakam


The biological activities of petroleum ether (P), 95% ethanol (E) extracts and hydro-distillation (H) of Perilla frutescens (Linn.) Britton leaves were evaluated. Total phenolic contents, anti-oxidant activity (DPPH and hydroxyl radical (OH) scavenging) and the anti-inflammatory effects were determined. The volatile components of the hydro-distillation were determined by GC-MS analysis. The stability of cosmetic products containing the extract and dermal irritancy on human skin were also evaluated. The results suggested that the total phenolic content in E extract was higher than that in P extract.  All extracts presented significantly inhibited hydroxyl radical induced deoxyribose degradation with an IC50 value in the range of 0.045±0.017-0.092±0.024 mg/ml more than Trolox (IC50=1.351±0.408 mg/ml). For DPPH assay, E extract showed significantly (p<0.05) higher activity than P extract. The highest ability of inhibition exhibited in E extract was 040E (IC50=0.824±0.061 mg/ml) which is also related to GEAC (3.298±0.237 mg/g extract) and TEAC (13.002±0.933 mg/g extract) values. The anti-inflammatory effects of all E extracts at low concentration 0.0625 ug/g were higher activity to NO inhibition than those of all P extracts. GC-MS analysis showed the presence of 2-Hexanoylfuran and 1-Pentanone in all three samples. The irritation on the volunteer’s skin was not found. To conclude, P. frutescens leaves showed antioxidants activity, serving as an anti-inflammatory agent, and volatile compounds (essential oil) which might be a good plant for cosmeceutical products.


Anti-inflammatory, Antioxidant, Cosmetic product, skin irritation, stability test

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