Isolation of Acidotolerant/Acidophilic Photosynthetic Bacteria from Peat Soils in Nakhon Si Thammarat Province and the Promoting of Biomass and 5-Aminolevulinic Acid Pr

Angkana Saikeur

Abstract


Peat soils samples collected from 7 districts of Nakhon Si Thammarat province were used for isolation acidotolerant/acidophilic photosynthetic bacteria. The soil samples were inoculated into GMY medium with pH of 4.5-6.5, and cultivated under microaerobic-light condition (5,000 Lux) at room temperature (32±3C) for 48 hours. The results showed that 9, 5 and 2 isolates can be isolated from the medium with pH of 5.5, 6.0 and 6.5, respectively. At lower pH values, 4.5 and 5.0, only 2 isolates were isolated. All isolates were subsequently screened for the production of 5-aminolevulinic acid (ALA) by culturing in the GMY medium, pH 6.0. The highest ALA (20.42 µM) was found from the isolate named Rhodopseudomonas palustrisJP255. The Plackett-Burman design with 7 factors (glycine, succinate, propionic acid, magnesium chloride, levulinic acid, pH and glucose) at 3 levels was performed for optimization biomass and ALA production of the JP255. By this design, approximately 218.78 µM ALA and 4.156 g/L biomass could be enhanced.

 

ละภัณฑ์ and biomass production of the JP255 LAuld be enhanced.vulinic acid (ALA) by culturing synthetic bacteria. ือว่าเขาแนะนำ Statistical analysis of the results from Plackett-Burman revealed that magnesium chloride, glucose and the pH gave positive affected on biomass and ALA production; whereas glycine succinate propionic acid and levulinic acid gave negative results. The suitable medium (1 liter) for ALA and biomass production of the JP255 was GMY medium (L-glutamic 3.8 g, DL-malic acid 2.7 g, yeast extract 2.0 g, (NH4)2HPO4 0.8 g, KH2PO4 0.5 g, MgSO4· 7H2O 0.2 g, CaCl2·2H2O 0.053 g, MnSO4· 7H2O 1.2x10-3g, thiamine-HCl 1.0x10-3g, nicotinic-HCl 1.2x10-3g and Biotin 1.0x10-5g) addition with glycine 5 mM, succinate 10 mM, propionic acid 1 mM, MgCl2· 6H2O 20 mM, levulinic acid 10 mM and glucose 10 mM and adjusted the final pH to 6.5.

Keywords


photosynthetic bacteria, 5-aminolevulinic acid, Plackett-Burman

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SCIENCE  AND  TECHNOLOGY  RMUTT  JOURNAL
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